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PhycoTech - Algal (Phytoplankton & Periphyton), Zooplankton, MacroInvertebrates, & Bacteria Analysis Services

Additional Options

High Particulate Phase
HIgh Particulate Epigreen
Images above demonstrate how we utilize fluorescence to locate algae in a high particulate sample. Both images taken at 400x. Left image is phase contrast, right image is green light excitation.

All reports and forms available in Adobe PDF format Adobe Acrobat (PDF) Format

Our Services

PhycoTech specializes in the analysis of lake, reservoir, stream, well, and marine samples for suspended and attached algae, zooplankton, macroinvertebrates and bacteria. We also provide algal analysis for fish and macroinvertebrate guts, as well as diatom and akinete composition in sediment cores. We have been providing high quality aquatic sample analysis for over 23 years with Dr. St. Amand having over 28 years experience in the field. Our customers vary from governmental agencies, private industry and universities, to lake owners associations and individuals concerned about local water quality. PhycoTech has processed over 33,000 biological samples to date.

We lead the industry by providing our customers with a wide variety of associated services such as specialized bioassays, photo documentation, data analysis, report generation, field study design, nutrient analysis and chlorophyll a analysis, all based on the most current technology. PhycoTech is the only commercial lab in North America to utilize a unique proprietary permanent mounting technique. These mounts allow you to get further data at a later date, as well as maintain a permanent archive of the sample that is easily stored, maintains fluorescence, and does not degrade with time. Permanent algal mounts allow archiving of diatoms and soft algae. Zooplankton mounts are stained and then permanently mounted for easier identification.

HPMA Mount
HPMA Permanent Algal Mount provides optically clear archival slides
Permanent Zooplankton Mount
Permanent Zooplankton Mount
Naphrax mount
NAPHRAX mount of acid cleaned diatoms, with room on the right side of the coverslip for voucher and QA/QC information

ASA System

PhycoTech's revolutionary, proprietary data management software, the ASA System

Nomarski
Legend: Nomarski and Fluorescent micrograph at 1000x showing single celled blue-green picoplankton.

Protocols and Microscopy Equipment

The HPMA method for producing algal sample slides provides an optically clear background while permanently infiltrating and preserving the sample for archival purposes (See Technical Approach for general mounting method, QA/QC and counting protocol). We have adapted this method, through proprietary changes, to algae, zooplankton and bacteria. Mounting distortion is minimal and the method provides the advantage of being able to go 100x to 1000x on the same algal or bacterial specimen. Zooplankton can be viewed up to 200x. Wet sample is always maintained in case clarification of identification is necessary. We strongly encourage our customers to use glutaraldehyde (final concentration of 0.25-0.50%) for preservation of algal samples. It offers minimal distortion and allows the use of epifluorescence on the sample while counting, which can dramatically improve the final results. Zooplankton and macroinvertebrate samples should be preserved in 70% ethanol.

PhycoTech, Inc. has assembled and maintains a comprehensive library of keys and taxonomic references to assist in providing the highest quality and technically accurate product. In addition to these books, the PhycoTech reference library includes hundreds of papers from the primary journals.

Microscopes used to process all samples include Olympus BHT and BMX, research-grade compound microscopes equipped with Nomarski optics (100x, 200x, 400x, and 1000x), Phase Optics (400x, 1000x), epifluorescence, and a trinocular head for photography. We've just added a new digital camera as well, allowing us to provide real-time image documentation. We also have a dissecting microscope available for macroinvertebrates and large zooplankton. Our compound microscopes are equipped with green, blue and UV excitation cubes, allowing us to use not only algal autofluorescence, but also stains that include Acridine Orange, Acriflavin, DAPI and Calclafluor White. Also available is Scanning Electron Microscopy through the Notre Dame Electron Microscopy Laboratory.

Data Management

Data Management Software

PhycoTech is now utilizing its proprietary data management software, the ASA System. The new program not only tracks samples from receipt to data delivery within the same software program, but also provides significantly more information for each sample. With ASA, we are able to provide not only biovolume estimates, but volume and surface area estimates as well. We will also now be providing data summaries on phyla, division or class level automatically, depending on the analysis requested. In addition, our new program has the capability to calculate over 89 different diversity indices and summary statistics, including Shannon, Maragalef and Berger-Parker Diversity measures, Species Richness and Evenness, Pollution Tolerance for diatoms and invertebrates, Environmental Tolerance for algae, Siltation Index for diatoms, Palmer Index, Nyggards Quotients, ACC:CMN for diatoms, and EPT for invertebrates, in addition to others. All taxonomic information from organism down to coloniality and structure will be provided in the data set.

PhycoTech, Inc.
620 Broad Street, Suite 100
St. Joseph, MI 49085, USA
Tel: 1-269-983-3654
Fax: 1-866-728-5579 / 1-269-983-3653
Email: info@phycotech.com

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