- Read the Material Safety Data Sheets (MSDS) and all instructions prior to the usage of glutaraldehyde (25% in water). Eye protection and gloves are required for the usage of glutaraldehyde. Glutaraldehyde is a toxic chemical and must be treated as such. Use glutaraldehyde only in a well ventilated area or under a chemical fume hood.
- Pour homogenized sample into a 250 mL amber poly bottle (or similar bottle), leave 3-5 cm headspace from the top of the bottle mouth.
- Keep the sample in a cooler until the sample can be preserved. Samples can be held in a dark, cool container for several hours.
- Add 1.0mL of 25% aqueous general grade glutaraldehyde for each 100mL of algal sample which is to be preserved. The final concentration of glutaraldehyde in the sample should be 0.25-0.50%.
- Securely tighten the lid onto the sample bottle.
- Shake the sample bottle vigorously 5 times.
- Uncap the sample and check for a thin layer of white foam or bubbles on the surface of the sample.
- If the glutaraldehyde is evident in the sample, tightly close the lid onto the sample bottle. The sample is now preserved.
- If the glutaraldehyde can not be detected in the sample, additional glutaraldehyde might be necessary. Normally, additional preservative is needed only for extremely dense bloom or tow samples or dense periphyton samples where you are dealing with an algal slurry. Add the additional glutaraldehyde (in maximum increments of .25mL of glutaraldehyde per 100 mL of sample) to sample bottle. Repeat steps 5-7 until sample preservation is achieved.
- Store the sample in a cool, dark place (cover with aluminum foil) until it can be processed.
Keep the glutaraldehyde bottle tightly closed and stored in a well ventilated area when not in use!
PhycoTech will not accept any responsibility for injuries or damage incurred due to the use, misuse, or storage of glutaraldehyde.